《植物生理学报》 2009, 45(10): 981-985
通信作者：田惠桥；E-mail: hqtian@xmu.edu.cn；Tel: 0592-2186486
摘 要：
采用植物组织培养、解剖学及组织化学定位方法研究太子参试管微块根发育的形态结构与营养物质积累特征的结 果表明: 太子参微块根由组培苗膨大的腋芽基部长出的不定根发育而成, 经历了初生结构与次生结构发育, 其膨大加粗是 由于不定根的次生生长。维管形成层向内形成大量的次生木质部构成微块根的主要部分。淀粉粒是太子参微块根的主要 营养存储方式。随着微块根的次生生长, 淀粉粒先在次生木质部薄壁细胞中形成, 随后在次生韧皮薄壁细胞中也大量积累。 膨大的微块根可以合成太子参皂苷, 成熟微块根中次生韧皮部的皂苷含量略高于次生木质部。离体太子参微块根的生长发 育和营养物质的积累与块根中的相同。关键词：太子参; 微块根; 组织化学; 淀粉粒; 皂苷
收稿：2009-08-19 修定：2009-09-23
资助：厦门市科技计划项目(3502Z20084037)和宁德市科技计划项目(20090024)。
Corresponding author: TIAN Hui-Qiao; E-mail: hqtian@xmu.edu.cn; Tel: 0592-2186486
Abstract:
The feature of morphology and structure of in vitro micro-root tubes of Pseudostellaria heterophylla Miq. and their nutrition accumulation were studied using anatomy and histochemistry methods. The micro-root tubers came from inflated adventitious roots of axillary bud. The secondary growth of micro-root tubers made them enlarge. Its vascular cambium formed a large number of xylem parenchyma cells which consisted of main structure of the tuber. The micro-root tubers synthesized starches during its enlargement. Starches were the mainly storage material in the micro-root tubers, which were stored in xylem parenchyma cells. With the secondary growth of micro-root tubers, starches were firstly formed in secondary xylem parenchyma cells, and then abundant starches were also accumulated in most secondary phloem parenchyma cells. In later microroot tubers, the xylem and phloem parenchyma cells synthesized saponin materials which could be detected using a histochemical method. The development and nutrition accumulation of in vitro micro-root tubers of P. heterophylla are as same as in vivo, which will provide an assay system of P. heterophylla to investigate its tuber development and application.Key words: Pseudostellaria heterophylla; micro-root tuber; histochemistry; starches; saponin
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